High performance liquid chromatography (HPLC) and ultra performance liquid chromatography (UPLC) are common techniques used in analytical laboratories to separate components. HPLC and UPLC separate different components found in mixtures by utilizing high pressure to drive solvents through columns. Despite its common purpose, there are some differences between both. In this blog, we explore the difference between HPLC and UPLC, shedding light on its importance in the analytical field. 

HPLC Vs UPLC: What are the Similarty in HPLC and UPLC? 

Both these methods are used to separate components found within a mixture. These liquid chromatography methods use liquid solvents in the mobile phase. They are employed to identify and quantify components. 

What is the Difference Between HPLC and UPLC? 

Here are some of the major difference between HPLC and UPLC: 

• Pressure: The key difference between HPLC and UPLC is the pressure it can withstand. Earlier known as high-pressure liquid chromatography, it can withstand pressure up to 6,000 psi, while UPLC can withstand pressure upto 15,000 psi. 

• Particle Size: Along with high pressure levels, UPLC also has a higher sensitivity. This means it allows a lower particle size within the columns, making it ideal for low-component analysis. 

• Applications: Due to its high sensitivity, ultra performance liquid chromatography is used for dietary supplements and biological samples that have low quantities of different components. At the same time, HPLC is employed in applications like water purification and the identification of impurities in pharmaceuticals. 

• Flow Rate: UPLC uses a higher flow rate than HPLC due to its small particle size and higher pressure.

• Sensitivity and Resolution: UPLC’s smaller particle size and less band broadening provide higher sensitivity and superior resolution. 

Conclusion 

High performance liquid chromatography and ultra performance liquid chromatography are advanced analytical methods used to separate and evaluate components of different mixtures. Aral Research offers UPLC and HPLC analysis services to life science and pharmaceutical fields. Our experienced team is well-equipped to develop the HPLC method for chiral and achiral molecules. We provide customized solutions, quick turnaround time, and competitive pricing to support and meet analytical goals. Call us to discuss your requirements.