Purity by HPLC/UPLC Services


Purity by HPLC/UPLC Services

Aral Research offers a comprehensive range of High Performance Liquid Chromatography (HPLC) and Ultra Performance Liquid (UPLC) analysis services to pharmaceutical and life science related fields. We provide a range of purity through HPLC/UPLC services to support drug discovery and development. We employ HPLC/UPLC to analyse drug compounds for  pharmaceutical research and life sciences. Our team is well-equipped to develop the HPLC method for chiral and achiral molecules. At Aral Research, our analytical scientists are highly experienced and accomplished in HPLC and UPLC expertise to support research, method development, and validation projects. Our client approach and industry experience enable us to bring our expert guidance for drug discovery and development. We strive to align with your drug development goals through flexibility and iterative processes for swiffer turnaround. High Performance Liquid Chromatography procedure validation ensures proper sensitivity, selectivity, and reproducibility.


High Performance Liquid Chromatography (HPLC) and Ultra Performance Liquid (UPLC) are liquid chromatography methods to separate and detect compounds found in complex matrices. HPLC operates at lower pressure viz max. <6000 psi, and UPLC operates at higher pressure viz ~15000 psi for smaller particle sizes in columns. Aral Research has extensive experience in analytical chromatographic separation from simple to complex projects.

We employ HPLC/UPLC to drug test the formulation for stability, concentration, purity, and homogeneity. Our clients benefit from our expertise and experience in HPLC analysis services. We provide tailored solutions, fast turnaround time, and competitiveness to meet your analytical goals.

Method Development and Validation Services

At Aral Research, our method development and validation services utilising High Performance Liquid Chromatography (HPLC) are meticulously tailored to meet the stringent requirements of pharmacopeial standards and the diverse needs of pharmaceuticals, active pharmaceutical ingredients (APIs), and natural products. Leveraging our expertise, we design and optimise HPLC methods for the precise analysis of new drugs, APIs, and natural compounds, ensuring optimal separation, identification, and quantification. Our approach integrates cutting-edge instrumentation and advanced analytical strategies to address the complexities inherent in pharmaceutical analysis. Additionally, our validation processes adhere strictly to pharmacopeial guidelines, guaranteeing the accuracy, reliability, and compliance of our methods. Through comprehensive validation protocols, we meticulously assess parameters such as specificity, precision, accuracy, linearity, and robustness, providing clients with confidence in the integrity and regulatory compliance of their analytical methods.

Aral Research’s core focus is to make drug development a great success through our extensive experience in HPLC/UPLC development method offerings. Our team of scientists and analytical experts emphasises productive, long-term collaboration. Our UPLC presents the possibility to expand and extend the utility of chromatography in clinical trials, routine quality screens, metabolomics studies, and food safety analyses. HPCL is a powerful analytical technique for separating, identifying, and quantifying components in the mixture. We provide a variety of HPLC/UPLC methods with different detectors for analytes: Mass Spectrometry, LCMS, Refractive Index (RI), UV (ultraviolet) or VIS (Visible), Charged Aerosol Detectors (CAD), and Light Scattering.

Why Choose Our Purity by HPLC/UPLC Services?

HPLC/UPLC Analysis in Pharmaceutical Analysis

Evaluates the pH, temperatures, mobile phase, gradient profiles, and column chemistries to ensure a reliable and robust separation of the sample of interest, reducing time in method development and validation.

HPLC/UPLC Analysis in Pharmacokinetic & Bioequivalence Studies

Selectivity and sensitivity of UPLC at low-detection levels allows for reliable and accurate data that can be used for statistical pharmacokinetic analysis.

HPLC/UPLC Analysis in Biometabolic Studies

Ultra Performance Liquid Chromatography is used for reproducible, accurate, and reliable analysis of amino acids in protein characterization, nutritional analysis of food, and cell culture structure.

HPLC/UPLC Analysis in Toxicity Studies

UPLC’s high resolution allows for precise detection of candidate drugs for inhibition of metabolising enzymes and toxicity. Its sensitivity allows detection even at lower concentrations.


Have any questions?

Frequently Asked Questions


High Performance Liquid Chromatography (HPLC) is a high pressure liquid chromatography method. It is widely used in analytical laboratories due to its outstanding sensitivity and peak resolution. HPLC systems can handle pressure upto 6000 psi (pound square inch).

Ultra Performance Liquid Chromatography (UPLC) is the latest advancement in liquid chromatography for separation, identification, and quantification. It is renowned for chromatographic efficiency, quick turnaround time, and sensitivity. A UPLC system can handle pressure upto 15000 psi.

HPLC (High Performance Liquid Chromatography) and UPLC (Ultra Performance Liquid Chromatography) are the liquid chromatography methods. They are used to separate components of a mixture or compound using high pressure to push down solvents through columns. UPLC column particle size is less than 2um, while HPLC column particle size is 5um.

The principle of HPLC is the destruction of the analyte (sample of interest) between the mobile and stationary stages. Based on the sample's chemical structure, the molecules are retarded when passing the stationary phase. The intermolecular interaction between the sample and packing material determines the time on the column. In this, different constituents of the analyte are eluted at different times. Hence, the separation of sample ingredients.

HPLC is a powerful liquid chromatographic technique that is used in diverse fields, such as pharmaceutical, clinical, forensic science, drug development, and bioanalytical.

Ultra High Performance Liquid Chromatography (UHPLC) is a liquid chromatography technique similar to HPLC. It is used to separate different constituents of a compound or mixture. It is widely used to identify, quantify, and separate compounds by pushing solvents through a column using high pressure.

Some of the typical HPLC/UPLC analyses include:

  • Impurity analysis
  • Peptide mapping & sequencing
  • Target characterization & identification
  • Metabolomics
  • Toxicology screening
  • Antibody analysis
  • API Quantitation
  • Method development & validation

What our client have to say


K. K. Dokania WordPress Dev.

Aral Research committed to providing with the highest level of service and qualitative analytical data to our client. Our team of experts takes pride in staying up to date with the latest techniques and methodologies, and we are dedicated to providing innovative and efficient analytical solutions to meet your research needs.

Amelia Ava WordPress Dev.

ARAL RESEARCH provide qualitative analytical data with proper interpretation to help you with your research needs. We specialize in characterization reports that utilize various analytical techniques such as Mass, LCMS, HPLC, and NMR. We are proud to offer customized reports tailored to your specific research requirements.