Mass Spectrometry for Protein Analysis

Posted on November 2, 2024November 2, 2024 by aralresearch_admin

Mass spectrometry (MS) is a primary analytical method for protein research and the study of biomolecules. Mass analysis of proteins measures ions’ mass-to-charge (m/z) ratio to identify and quantify molecules in simple to complex mixtures. The development of high-throughput mass analysis has made this analytical method profound across different fields, particularly proteomics. This further expanded research related to protein structure, function, modification, and dynamics.

What is Protein Mass Spectrometry?

Proteomics is the study of proteins in biological systems such as tissues, cells, or organisms. Technological advancements in mass spectrometry have helped open horizons related to proteomics. Protein mass spectrometry is the use of mass spectrometry in studying and characterizing proteins, comprising quantification, interaction mapping, profiling, and identification of post-translational modifications. Aral Research provides mass analysis in Ahmedabad backed with a WATERS SDQ detector and TQD detector equipped with Electrospray Ionization (ESI) and Atmospheric-pressure Chemical Ionization (APCI), enhancing MS capabilities.

Application of Mass Spectrometry for Protein Analysis

Identification and verification of proteins.
Determining protein structure, function, folding & interactions.
Identification of protein from the mass of its peptide fragments.
Quantitate proteins (absolute or relative) in a given sample of interest.
Evaluate enzyme reactions, protein digestion and chemical modification.

MS Workflow for Protein Complex Research

AQUA Strategy: The Absolute Quantification of Ubiquitination (AQUA) strategy involves developing synthetic heavy peptides that demonstrate ubiquitination linkages. The peptides are blended with the concentration of sample peptides and analyzed by mass spectrometry.
Labelling of Protein Extracts: Proteins from different cell lines are individually labelled with isobaric labels. Following the breakdown, the peptides from every sample are combined and analyzed by MS, allowing for every sample’s peptides to have a similar mass-to-charge ratio.
Independent Examinations: Samples are evaluated independently, and the cell lysate extracts the needed protein complex with affinity purification. Following digestion, the complex is examined using mass analysis to determine its components.

Protein Mass Spectrometry at Aral Research

Our mass analysis, coupled with HPLC service in Ahmedabad, aims to empower researchers with precise and reliable data with state-of-the-art techniques and equipment to characterize biomolecules, emphasizing proteins and peptides, metabolites, lipids and small molecules mass spectrometry. Backed with ESI and APCI, we assure precision and high-quality analytical data for your diverse requirements. Call us to discuss your analytical needs.

Unlocking the Power of Mass Analysis: Key Benefits for Modern Research

Posted on September 25, 2024September 26, 2024 by aralresearch_admin

Modern research demands powerful and efficient analytical techniques and methods to delve into the deeper and intricate aspects of compounds. Mass analysis is a cutting-edge analytical method that has revolutionized the field of research, enabling researchers and scientists to get invaluable insights into molecular weight, structural characteristics, and analysis. So, let’s explore the key benefits of mass analysis in modern research, emphasizing its importance in various scientific fields.

Understanding Mass Analysis

Mass analysis or mass spectrometry is a powerful analytical method that measures the mass-to-charge ratio (m/z) of ions to identify and quantify molecules. This analytical method involves the ionization of analytes and separation based on the mass-to-charge ratio utilizing the mass analyzer. In recent years, MS analysis has been employed to discover new molecules and determine quantities of known components and structural & chemical properties of molecules in industries such as pharmaceutical, chemical, biotechnology, food & beverages, and the life science industry. Aral Research offers impeccable mass analysis in Ahmedabad. Our MS spectrometry solutions are designed to deliver precise results, high-quality data, analytical flexibility, and real impact on your research.

Benefits of Mass Analysis for Modern Research

High Sensitivity & Precision

Mass spectrometry is utilized to determine the structural aspects of molecules, helpful for researchers working on environmental, metabolomics, and proteomics. It is used to examine the molecular weight, elemental content of chemicals, and fragmentation of patterns. These valuable insights are very helpful in understanding the structure of biomolecules and chemical reactions. Moreover, mass analysis is also used for the identification of proteins, lipids, and peptides.

Quantitative Analysis

Another advantage of mass spectrometry is that it can be used for qualitative analysis to determine the concentration of particular compounds in a sample of interest. This helps in encompassing nature’s biological process and variations within the metabolite formations.

Highly Versatile

Mass analysis is a versatile analytical method that can be applied in different fields. It finds applications in fields such as biochemistry, chemistry, forensics, pharmaceutical, clinical research, life science, and environmental science. Mass spectrometry has become an indispensable tool in modern research disciplines as it can analyze all types of molecules.

Integration with Latest Technology

The emergence of more advanced and powerful mass spectrometers has expanded the capabilities is mass analysis. It provides a more enhanced sensitivity, precision, and quick analysis. Analytical techniques like gas chromatology and ion mobility integration with mass analysis have made this analytical technique more versatile and robust.

Mass analysis is an indispensable tool for the analysis of a range of range samples, providing insights into the structure, concentration, isotopic distribution, and biological molecules in the sample. Aral Research also provides LCMS analysis in Ahmedabad for high sensitization quantification and qualitative workflow for research and development. LCMS integrates the power of mass spectrometry and liquid chromatography, providing more comprehensive data. For more details on mass analysis services, call us today.

Visit:- How can Mass Spectrometry be used to Identify Unknown Compounds?

Mass Spectrometry & Its Importance for the Analysis & Discovery of Active Molecules in Natural Products

Posted on July 26, 2024July 26, 2024 by aralresearch_admin

Mass spectrometry (MS analysis) is considered the best method for analyzing a molecule’s structure. MS analysis offers details about a compound’s molecular weight and presents the atomic mass units and 10000 atomic mass units, depending on the accuracy of the mass analyzer. Additionally, mass spectrometry provides information related to analyzing compounds from natural products. In this blog, we discuss mass spectrometry and its importance for the analysis and discovery of active molecules in natural products.

Role of MS in Analysis & Discovery of Active Molecules in Natural Products

The development of mass spectrometry, along with research, has proven to be game-changing in enhancing the ionization process and ion analysis. Mass spectrometry has significantly contributed to qualitative control of natural products and to identifying new metabolites. Natural products consist of a mixture of complex quantity components. In the field of natural products, various studies regarding secondary metabolites have been validated through mass analysis.

Mass Spectrometry and Natural Products

The success of mass spectrometry over the years, along with variability methods and instruments, allows all molecules of natural products to be analyzed quantitatively and qualitatively.

Essential Oils: These oils are basically volatile metabolites, so the study and research are straightforward. Mass spectrometry allows the investigation of molecules that form these natural products.
Fatty Acid: Many plant specimens are composed of saturated and unsaturated fatty acids. Mass spectrometry, along with chemical derivational techniques, is used for research purposes to form ethyl ester and methyl.
Aromas & Flavours: The odour and flavour of fruits and vegetables are vital to distinguishing their taste and texture. Mass spectrometry is crucial to identifying the chemistry of these components. Many of these molecules are volatile and hence impossible to remove in the vapour stream. They are removed with non-polar solvents to introduce them to the chromatographic system.
Alkaloids: These are active ingredients that contain nitrogen in their structural features. Some alkaloids, such as nicotine, caffeine, and xanthine alkaloids, are directly analyzed by mass spectrometry, while others, with high volatility, need separation in gas chromatography.

Mass spectrometry analyzes natural products. Its high sensitivity and detailed structural information make mass analysis indispensable in research and development laboratories. Aral Research offers high-quality mass spectrometry analysis services. We ensure high-quality mass analysis data with a WATERS SDQ detector and TQD detector equipped with Electrospray ionization (ESI) and Atmospheric-pressure Chemical Ionization (APCI) mode detection. We serve many industries, including proteomics, forensics, metabolomics, and life science. We can help you solve analytical problems and research goals.

How can Mass Spectrometry be used to Identify Unknown Compounds?

Posted on July 18, 2024July 26, 2024 by aralresearch_admin

Mass spectrometry is an instrumental analytical technique to measure the mass-to-charge ratio of one or more molecules present in a sample of interest. It is a powerful analytical tool to quantify unknown materials and identify unknown compounds and structural and chemical properties of different molecules.

Working Principle of Mass Spectrometry Can Be Used to Identify Unknown Compound

Mass spectrometry, with its ability to measure the mass-to-charge ratio (m/z) and calculate the exact molecular weight of sample components, is a versatile tool. It identifies unknown compounds through molecular weight determination, quantifies familiar compounds, and determines structure and chemical properties. It essentially studies the effects of ionizing energy on molecules, generating multiple ions from the sample, then separating them according to their specific mass-to-charge ratio (m/z), and noting the related abundance of each type of ion. This versatility makes it an intriguing field of study.

Component of Mass Spectrometry

Ionization source
Mass analyzer
Ion detection system

Here is How Mass Spectrometry Can Be Used to Identify Unknown Compound

Ionization Source: At first, the molecules are converted to gas-phase ions to move them and are manipulated by external electric and magnetic fields. The molecular ions undergo fermentation, and each primary product derived from the molecular ion also undergoes fermentation.
Mass Analyzer: After ionizing, the ions are sorted and separated based on mass-to-charge (m/z) ratios and detected proportionally to their abundance.
Ion Detection System: The separated ions are later measured and out through a data system where the mass-to-charge ratio is stored together with its relative abundance. A mass spectrum of the molecule displays the result in the form of a plot of ion abundance Vs. m/z ratio. The ions offer information about the precursor molecule’s nature and structure. In the mass spectrum, in the case of a pure compound, if a molecule ion is present, it appears at the highest value of m/z, later followed by ions consisting of heavier isotopes, and gives the compound’s molecular mass.

Aral Research is a full-fledged research lab specializing in custom analytical services for complex compounds. We aim to provide researchers with modern techniques and equipment to characterize biomolecules. We are equipped with the latest mass spectrometry equipped backed with Electrospray Ionization (ESI) and Atomspheric-pressure Chemical Ionization (APCI) mode detection. Contact us to discuss your requirements.

What is Mass Spectrometry?

Posted on June 20, 2024July 2, 2024 by aralresearch_admin

Mass spectrometry is a technique used to precisely determine the mass of different molecules in a sample of interest. This is done with a mass spectrometer device, which produces a mass spectrum read out by measuring a single mass spectra in a sample. In this blog, we closely examine mass spectrometry and how it works to shed light on its importance in various scientific research.

Everything You Need to Know About Mass Spectrometry

Mass spectrometry is an analytical method that uses ionization and mass analysis of compounds to determine the mass, formula, and structure of the sample of interest. It measures the mass-to-charge ratio (m/z) of one or more molecules present in the sample of interest. Mass spectrometers are employed to identify unknown compounds through molecular and chemical properties of molecules.

How Do Mass Spectrometers Work?

There are various methods used for mass spectrometry. There are three basic components of mass spectrometry analysis:

Molecule Ionization: The sample material is heated to the gas ion phase or combined with the matrix material. Molecules are ionized by employing an ion source, such as an electric field, chemical, or electrospray ionization, to produce charged ions.

Mass Analyzer: After the ions are ionized, they are separated according to their mass-to-charge (m/z) ratios. The mass analyzer records the speed and relative abundance of ions to provide a visual readout or mass spectra.

Ion Detection System: The separated ions are measured and transferred to a data system, where the mass-to-ratio and relative abundance are stored together. Different sample compounds have varying masses. The readout allows researchers to determine the sample’s composition by comparing the known element results.

Mass Spectrometry Analysis With Aral Research

Aral Research provides mass spec analysis services to identify complex moiety. Our mass analysis services aim to detect and quantify known and unknown compounds, mixtures, and chemical structures of different molecules to offer an accurate and quantitative analysis. Our analytical laboratory is equipped with a WATERS SDQ detector and TQD detector with Electrospray Ionization and APCI (Atmospheric -pressure Chemical Ionization) mode detection to support mass range upto 2000 m/z. Partner with the most trusted analytical laboratory in India for your research project.

Category: Mass Analysis