Mass spectrometry (MS) is a primary analytical method for protein research and the study of biomolecules. Mass analysis of proteins measures ions’ mass-to-charge (m/z) ratio to identify and quantify molecules in simple to complex mixtures. The development of high-throughput mass analysis has made this analytical method profound across different fields, particularly proteomics. This further expanded research related to protein structure, function, modification, and dynamics.
What is Protein Mass Spectrometry?
Proteomics is the study of proteins in biological systems such as tissues, cells, or organisms. Technological advancements in mass spectrometry have helped open horizons related to proteomics. Protein mass spectrometry is the use of mass spectrometry in studying and characterizing proteins, comprising quantification, interaction mapping, profiling, and identification of post-translational modifications. Aral Research provides mass analysis in Ahmedabad backed with a WATERS SDQ detector and TQD detector equipped with Electrospray Ionization (ESI) and Atmospheric-pressure Chemical Ionization (APCI), enhancing MS capabilities.
Application of Mass Spectrometry for Protein Analysis
- Identification and verification of proteins.
- Determining protein structure, function, folding & interactions.
- Identification of protein from the mass of its peptide fragments.
- Quantitate proteins (absolute or relative) in a given sample of interest.
- Evaluate enzyme reactions, protein digestion and chemical modification.
MS Workflow for Protein Complex Research
- AQUA Strategy: The Absolute Quantification of Ubiquitination (AQUA) strategy involves developing synthetic heavy peptides that demonstrate ubiquitination linkages. The peptides are blended with the concentration of sample peptides and analyzed by mass spectrometry.
- Labelling of Protein Extracts: Proteins from different cell lines are individually labelled with isobaric labels. Following the breakdown, the peptides from every sample are combined and analyzed by MS, allowing for every sample’s peptides to have a similar mass-to-charge ratio.
- Independent Examinations: Samples are evaluated independently, and the cell lysate extracts the needed protein complex with affinity purification. Following digestion, the complex is examined using mass analysis to determine its components.
Protein Mass Spectrometry at Aral Research
Our mass analysis, coupled with HPLC service in Ahmedabad, aims to empower researchers with precise and reliable data with state-of-the-art techniques and equipment to characterize biomolecules, emphasizing proteins and peptides, metabolites, lipids and small molecules mass spectrometry. Backed with ESI and APCI, we assure precision and high-quality analytical data for your diverse requirements. Call us to discuss your analytical needs.
